Part:BBa_K1492001:Design
Clickable Outer Membrane Protein y
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 72
Illegal NgoMIV site found at 405
Illegal AgeI site found at 823 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The protein has a TAG codon and HA-tag added at the C-terminus of the protein. The TAG codon is for the incorporation of a Non Natural Amino acid. With the proper tRNA synthetase any Non Natural Amino Acid can be incorporated into the protein and displayed on the membrane. The HA-tag was used for characterization of the protein. Both modifications have been made by using an overhang primer. After the modification the protein has been renamed to Clickable Outer Membrane Protein y (COMPy).
We as team TU Eindhoven 2014 state that we have improved the functionality and “ease of use” of BioBrick part: BBa_K811003. The functionality has been improved by adding the TAG. This allows the incorporation of any non-natural amino acid. The “ease of use” has been improved by the introduction of a HA-tag to the protein. This tag makes it a lot easier to screen whether or not the protein is expressed using fluorescent anti-HA antibodies.
Figure 1: Gene design of COMPy
Source
See BBa_K811003